RNA

Part:BBa_K3081115

Designed by: Yiwen Wang   Group: iGEM19_Peking   (2019-10-16)


a 20bp sequence targeting the R3(top strand) DnaA box on E.coli oriC

The 20 bp sgRNA targets to the R3+ DnaA box on E.coli genome replication initiation region, OriC. In natural situations, R3 is a low affinity box for DnaA binding. By blocking the binding of DnaA protein to R3 box, moderate arrest and inhibition of genome replication initiation is achieved. For more detailed information, see BBa_K3081058

T--Peking--box.png

Figure1: Different boxes related to E.coli genome replication.This figure is adapted from the review of Costa, A. et al[1]


Reference: [1]Mott, M. L. , & Berger, J. M. . (2007). Dna replication initiation: mechanisms and regulation in bacteria. Nature Reviews Microbiology, 5(5), 343-354.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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